KCNMB4 Works Great

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Thermo Scientific

Product Name:

KCNMB4 Antibody

Catalog Number:

PA5-50692

Clone Number:

N/A

MaxiK channels are one of the large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels are of 2 subunits: the pore-forming alpha subunit and the modulatory beta subunit. It controls the auxiliary beta subunit which slows activation kinetics, leads to steeper calcium sensitivity, and shifts the voltage range of current activation to more negative potentials than does the beta 1 subunit. Here we checked the time course effect of KCNMB4 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lysate

Primary Incubation

1:1000 dilution prepared in 1x TBST

Blocking Agent

5% milk with 1x TBST

Secondary Incubation

1:4000 dilution prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We found time dependent increase of KCNB4 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

This antibody is very good for western blot experiment

The Bad

None

The Bottom Line

This antibody works great and it is very specific. Highly recommended.

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