Great for STAT3 Activation Analysis

April 26, 2021

BlueskyReddit
Molecular Biology and Biochemistry
Med Uni Graz
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling

Product Name:

Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb

Catalog Number:

9145

Clone Number:

N/A

In our experimental project we determined downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigated the effect of LPA on inflammatory transcription factors in microglia cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

BV2 microglia cells

Primary Incubation

1:1000 overnight at +4

Blocking Agent

5% milk

Secondary Incubation

1:5000 goat anti-rabbit, 2 hours at room temperature

Tertiary Incubation

N/A

Detection

ChemiDoc Development System

Results Summary

LPA treatment induced activation of STAT3 in microglia cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Rather good signal but not that strong

The Bad

N/A

The Bottom Line

A good antibody for pSTAT3 detection

Share your experience with other scientists. Write a Review! »

Join the discussion