iNOS Immunofluorescence in Primary Microglia

October 08, 2020

Molecular Biology and Biochemistry
Medical University of Graz
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-iNOS antibody

Catalog Number:

ab3523

Clone Number:

N/A

The antibody was used in order to detect iNOS expression in untreated and LPA treated microglia cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Primary Murine Microglia

Primary Incubation

1:100

Blocking Agent

5% BSA

Secondary Incubation

1:200 Cy3 for 30 min at room temperature

Tertiary Incubation

N/A

Detection

Confocal Microscope

Results Summary

Treatment with 1µM LPA increased the expression of iNOS in primary microglia cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, reproducible results

The Bad

N/A

The Bottom Line

The signal was strong and clear without giving a high background.

Share your experience with other scientists. Write a Review! »

Join the discussion