ThermoFisher
CyQUANT™ LDH Cytotoxicity Assay
C20300
Info: Supplier Page
CATHa neurons were incubated for 24h with conditioned medium from control and treated microglia cells.
LDH assay kit
CATHa neurons
Perform multiple experiments with at least 6 replicates due to increased variation.
Conditioned medium from LPA treated microglia increased neuronal cytotoxicity. Use of different MAPK inhibitors abrogated the LPA induced effect.
https://doi.org/10.1186/s12974-020-01809-1
Easy and fast to use.
Rather high variation between replicates.
A simple colorimetric assay for determining cellular cytotoxicity.