Phospho Erk1/2 for Western Blot

October 28, 2020

BlueskyReddit
Molecular Medicine
TSRI
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb #9106

Catalog Number:

9106

Clone Number:

E10

This antibody produced too many nonspecific bands to be useful.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Lysates of human PC3 prostate cancer cells

Primary Incubation

1 ug/mL; 5% BSA in TBS-Tw20, ON, cold room

Blocking Agent

5% BSA in TBS-Tw20

Secondary Incubation

anti-mouse IgG HRP conjugate, 1:4,000 in 5%BSA-TBS-Tw20; 30 min, RT

Tertiary Incubation

None

Detection

Adnansta

Results Summary

This antibody produced too many nonspecific bands to be useful in detection phospho Erk1/2.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

We did not find anything good about this antibody.

The Bad

Too many nonspecific bands.

The Bottom Line

We would not recommend this antibody for detection of phospho Erk1/2.

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