Immunostaining for Frozen Sections

October 02, 2020

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling

Product Name:

YAP/TAZ

Catalog Number:

8418

Clone Number:

D24E4

Yaptaz antibody was used in embryonic cortex to determine the presence of yaptaz in blood vessels and in the other regions of the tissue

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Frozen embryonic mouse brain tissue (E13)

Primary Incubation

1:200, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Yap/taz antibody was used in E13 embryonic cortex to determine the presence of yaptaz in blood vessels and in the other regions of the tissue. The staining may appear nuclear or cytoplasmic ( or both) determining the localization of YAP/TAZ. Highly recommended for YAP/TAZ staining in cell lines, needs some standardisation in tissue samples

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific staining, good for thin sections

The Bad

Antigen retrieval may be necessary for better staining, concentrated antibody may give unspecific signals

The Bottom Line

The staining may appear nuclear or cytoplasmic ( or both) determining the localization of YAP/TAZ. Highly recommended for YAP/TAZ staining in cell lines, needs some standardisation in tissue samples

Share your experience with other scientists. Write a Review! »

Join the discussion