Immunostaining for Layer II/III marker in Brain Neocortex

October 01, 2020

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

Abcam

Product Name:

Cutl1/Cux1

Catalog Number:

ab54583

Clone Number:

N/A

CUTL1/CUX1 antibody was used to specifically label layer II/III neurons in the postnatal brain neocortex.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Frozen postnatal brain sections at P12

Primary Incubation

1:1000, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

CUTL1/Cux1 antibody is used determine the layer II/III positive neurons by immunostaining in P12 postnatal brain neocortex. With this antibody , it was however unspecific all over the cortex. This antibody may not be recommended for long term storage. More standardisation and higher dilutions may be effective for obtaining specific signal in the layer II/III region of the neocortex.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Works if standardized properly

The Bad

Antigen retrieval may be necessary for better staining, concentrated antibody may give unspecific signals, higher dilution required, long term storage may hinder the application

The Bottom Line

There are not many good CUTL1 antibody available. The antibody needs to be standardised at various steps.If other options are available, this may not be highly recommended.

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