Anti-phosph p38 MAPK for Western Blot

October 28, 2020

Molecular Medicine
TSRI
Assistant Professor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Phospho-p38 MAPK

Catalog Number:

9211

Clone Number:

N/A

The antibody 9211 against designed to identify the phosphorylated p#8 MAPK. However, the antibody bound to too many nonspecific bands in the lysates.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human prostate cancer cells, PC-3

Primary Incubation

1 ug/mL; 5% BSA in TBS-Tw20, ON, cold room

Blocking Agent

5% BSA in TBS-Tw20

Secondary Incubation

Anti-Rb IgG, HRP conjugate; 1:4,000; RT, 30 min

Tertiary Incubation

None

Detection

Adnansta

Results Summary

This antibody did not produce any obvious specific band across different conditions used for cell incubation prior to cell lysis.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

We did not find anything good about this antibody

The Bad

A lot of nonspecific band; no obvious p38 band, phosphorylated or not....

The Bottom Line

We would not recommend this antibody to detect phosphorylated p38 MAPK

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