Miltenyi
CD38 Antibody, anti-mouse, VioGreen™, REAfinity™
130-109-339
REA616
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Browse Similar Products: CD38 Flow Cytometry Antibodies
It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to titrate anti-CD38 that is expressed by B cells and not by T cells
Flow Cytometry
Wild-type mouse splenocytes
Surface staining with a-CD38 (1/200) (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Violet laser, Flow Cytometer
B cells are stained with a dilution at 1/200
It works well
Not as bright as other antibodies, but this can be good sometimes to avoid out of scale cells.