Miltenyi
CD2 Antibody, anti-mouse, APC-Vio 770, REAfinity
130-115-962
REA959
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It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to titrate anti-CD2 that is expressed in all T cells.
Flow Cytometry
Wild-type mouse splenocytes
Surface staining with a-CD2 (1/200) (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Red laser, flow cytometer
CD4 T cells are stained with a dilution at 1/200
It works
It can be used