Surface Staining of CD79b

Charite Strasse 1
Deutsches Rheuma-Forschungszentrum
Research Scientist

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Company:

Miltenyi

Product Name:

anti-mouse CD79b antibody APC-Vio 770, REAfinity

Catalog Number:

130-119-429

Clone Number:

REA1117

It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B lineage cells during autoimmunity. In this experiment, I wanted to titrate anti-CD79b that is expressed on B cells and not on T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Wild-type mouse splenocytes

Primary Incubation

Surface staining with a-CD79b (1/100) (20 min, 4° in the dark)

Blocking Agent

Clone 2.4G2 (Fc block)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Red laser, flow cytometer

Results Summary

B cells are stained with a dilution at 1/100.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

It works!

The Bad

N/A

The Bottom Line

It can be used for Flow Cytometry.

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