Neurofilament Immunofluorescence on Rat Sciatic Nerve Sections

September 30, 2020

Pharmacology
UCL
Postdoctoral Researcher

Overall

Quality of Results

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Company:

BioLegend

Product Name:

Purified anti NF-H/NF-M, Hypophosphorylated

Catalog Number:

835603

Clone Number:

SMI 35

Used to replace Eurogentec SMI-35 antibody which has been discontinued. Clear staining with little background in rat sciatic nerve (15μm cryosections)

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rat Sciatic Nerve Crysoections (15μm)

Primary Incubation

1:500, overnight at 4 degrees

Blocking Agent

Normal Horse Serum

Secondary Incubation

1:300, 1hr at room temperature

Tertiary Incubation

N/A

Detection

Confocal Fluorescence

Results Summary

Good staining, low background, reliable

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Reliable, Specific

The Bad

N/A

The Bottom Line

Recommended

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