Cell Signaling
P-AKT
4060
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Oxygen is a vital element and toxicity may not be immediately obvious. Moreover, impaired oxygen delivery in critically ill patients is associated with increased mortality. As a consequence, reassuring oxygen delivery has become a cornerstone of many resuscitation protocols and liberal use of supplemental oxygen is common.
Western Blot
Cell and mouse tissue lysate samples
1:1000 dilution in 2% skim milk prepared in 1X TBST
2% skimmed milk
1:4000 5% skim milk prepared in 1X TBST
None
ECL
We found the results time dependent increased P-AKt levels.
N/A
No background and very strong signal
Very strong signal.