IL1-beta for Western Blot

Department of Pharmacology and Toxicology
Augusta University
Postdoctoral Researcher

Overall

Quality of Results

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Company:

cell Signalling

Product Name:

IL1-beta

Catalog Number:

12703S

Clone Number:

N/A

Hyperoxia involves the administration of high concentrations of inspired oxygen to the lung. Hyperoxemia reflects high levels of oxygen in the blood.we found time dependent increase of IL 1 beta by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell and mouse tissue lysate samples

Primary Incubation

1:1000 dilution in 2% skim milk prepared in 1X TBST

Blocking Agent

2% skimmed milk

Secondary Incubation

1:4000 5% skim milk prepared in 1X TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

This antibody working very well and we found that time dependent increase by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

No background and very strong signal

The Bad

None

The Bottom Line

This antibody working very good and strong signal.

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