Goat Anti-Mouse IgG H&L for Western Blot

October 22, 2020

BlueskyReddit
Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Goat Anti-Mouse IgG H&L (HRP)

Catalog Number:

ab205719

Clone Number:

N/A

I use this antibody as the secondary antibody for Western blotting to detect primary antibodies raised in mouse. Here, I am showing the beta actin in THP1 cell samples treated with different compounds.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells treated with different compounds

Primary Incubation

1:10000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-mouse antibody 1:20000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

not applicable

Detection

ECL based detection

Results Summary

This anti-mouse HRP antibody is highly compatible with abcam primary antibodies.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

High sensitivity

The Bad

Nothing

The Bottom Line

This antibody works great for Western blotting.

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