Nice Bax (D2E11) Rabbit mAb for Western Blot

October 12, 2020

BlueskyReddit
Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Bax (D2E11) Rabbit mAb

Catalog Number:

5023S

Clone Number:

N/A

I determined the levels of Bax protein as a part of apoptosis examination in THP1 cells treated with different compounds.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells treated with different compounds

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

Not applicable

Detection

ECL based detection

Results Summary

I did see Bax expression induced by different compounds in THP1 cells.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High sensitivity

The Bad

Nothing

The Bottom Line

Overall, its a great antibody.

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