Cell Signaling Technologies
Caspase-8 antibody
4790S
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I used this antibody to detect total caspase 8 (as the loading control) in THP1 cells treated with different compounds.
Western Blot
THP-1 cells treated with different compounds
1:1000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking
Not applicable
ECL based detection
This antibody specifically detected total caspase 8 protein but not cleaved caspase 8. This, in conjuction with cleaved caspase-8 antibody can be used to determine the amount of active caspase -8.
Specificity
Some background and and non-specific bands were observed.
This antibody works but gives some background noise.