Cleaved Caspase-8 Antibody Works Well

October 26, 2020

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technologies

Product Name:

Cleaved Caspase-8 (Asp384) antibody

Catalog Number:

9748S

Clone Number:

N/A

I used this antibody to detect apoptosis in THP1 cells treated with different compounds.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells treated with different compounds

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-mouse antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

Not applicable

Detection

ECL based detection

Results Summary

The induced apoptosis differed between several compounds.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

High specificity and no background

The Bad

Nothing

The Bottom Line

This antibody works great for detecting cleaved caspase 8 protein in western blotting.

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