Cell Signaling Technologies
Caspase-3 antibody
14220S
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Browse Similar Products: Caspase-3 Western Blot Antibodies
I used this antibody to probe for total caspase 3 (pro-apoptotic protein) in THP1 cells treated with different compounds.
Western Blot
THP-1 cells treated with different compounds
1:1000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking
Not applicable
ECL based detection
Treatment with different compounds yielded altered levels of Caspase 3 protein.
It works well using manufacturer's recommended dilution
It gives some background on the blot.
Overall, this antibody serves the purpose of detecting total Caspase 3 protein in Western blotting.