Cleaved Caspase 3

October 09, 2020

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

Cleaved Caspase-3 (Asp175) Antibody

Catalog Number:

9664S

Clone Number:

N/A

I used this antibody to detect the apoptosis in THP-1 cells treated with different compounds.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells treated with different compounds

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

Not applicable

Detection

ECL based detection

Results Summary

Different compounds induced apoptosis in THP1 cells in various degrees.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

High sensitivity

The Bad

nothing

The Bottom Line

This antibody efficiently detects cleaved caspase 3 protein in Western blots.

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