Phospho-RIP (Ser166) Antibody for Western Blot

October 01, 2020

BlueskyReddit
Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

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Company:

Cell Signaling Technologies

Product Name:

Phospho-RIP (Ser166) antibody

Catalog Number:

65746S

Clone Number:

N/A

I used this antibody as an apoptotic marker in the untreated and stimulated THP-1 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Untreated and stimulated THP-1 cells

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking

Tertiary Incubation

not applicable

Detection

ECL based detection

Results Summary

Stimulated cells showed a clear reduction of phospho RIP suggesting reduced apoptosis upon stimulaiton.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

specificity

The Bad

nothing

The Bottom Line

This antibody works well for western blot-based detection of phospho RIP protein in THP-1 cells.

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