ß-Tubulin (9F3) Rabbit mAb (HRP Conjugate) for Western Blot

October 02, 2020

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technologies

Product Name:

β-Tubulin (9F3) Rabbit mAb (HRP Conjugate)

Catalog Number:

5346S

Clone Number:

N/A

I used this antibody as a loading control for my samples stimulated with different compounds of interest.

Experimental Design and Results Summary

Applications

Western Blot

Sample

THP-1 cells stimulated with different compounds

Primary Incubation

1:1000 dilution, 18 hours at 4 degrees with gentle shaking

Blocking Agent

5% non-fat dry milk in Tris buffer containing 0.1% Tween-20

Secondary Incubation

none

Tertiary Incubation

Not applicable

Detection

ECL based detection

Results Summary

Beta tubulin works great as a loading control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Specificity

The Bad

Nothing

The Bottom Line

This HRP-conjugated antibody works great for western blot.

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