Cell Signaling Technologies
PI3 Kinase p110α antibody
4249S
C73F8
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Browse Similar Products: PI3 Kinase Western Blot Antibody Antibodies
I used this antibody to determine the endogenous levels of PI3 Kinase protein in human cancer cell lines. PI3 Kinase is one of the critical kinases that drives cancer cell proliferation and tumor development.
Western Blot
Human cancer cell lines: Hela, MCF7 and SiHa
1:1000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking
not applicable
ECL based detection
This antibody worked exceptionally well to detect the PI3 Kinase protein in cancer cells by Western blotting.
high specificity and no background
nothing
In total, a great antibody from Cell signaling Technologies for Western blotting.