CD11b Antibody Has Efficient Staining and Low Cost

Microbiology & Immunology
University of Rochester Medical Center
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP/Cyanine5.5 anti-mouse/human CD11b antibody

Catalog Number:

101227

Clone Number:

M1/70

In immunology, CD11b is very useful in defining of innate immune cells because many of them express high levels of CD11b including macrophages, granulocytes and monocytes. For example, some macrophages in many tissues can be defined as CD11b+ F4/80+, neutrophils as Ly6G+ CD11b+, inflammatory monocytes as Ly6C+ CD11b+ Ly6G-. CD11b antibody (M1/70) from Biolegend is an excellent antibody and PerCP/Cy5.5 is compatible with many other fluorophores in flow cytometry including FITC, green laser-excited, red laser-excited and blue laser-excited fluorophores. One more good thing is that Biolegend provides a small size of antibodies.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

mouse, innate immune cells including macrophages, granulocytes, monocytes, some DCs

Primary Incubation

0.1 ug in 100 ul of PBS containing 5% FBS, up to a million cells, 30-60 min at 4 degree

Blocking Agent

PBS + 5% FBS

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Flow cytometry

Results Summary

PerCP/Cy5.5 conjugated CD11b antibody from BioLegend stains innate immune cells very efficiently and brightly.

DOI or PMID #

PMID: 32747818

Additional Notes

N/A

Related Categories

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Summary

The Good

Efficient staining with low cost.

The Bad

Not really

The Bottom Line

CD11b antibody from BioLegend is reliable and consistent. PerCP/Cy5.5 conjugation allows multi-color flow cytometry.

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