Abcam
Tbr2 antibody
ab23345
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Tbr2 Anitbody was used to specifically mark intermediate progenitors of a developing neocortex at E12.5 stage of embryonic development.
Immunofluorescence
Frozen embryonic mouse brain tissue (E12)
1:400, 4 degrees overnight
10% Normal donkey serum in PBST (0.5% Triton X 100)
1:500, AF 488
None
Basal/Intermediate progenitors from the E12.5 developing mouse cortex were specifically labelled with this antibody. Antigen retrieval using Na.Citrate buffer (samples cooked in microwave with the buffer-2-3 min boiling) improves the staining significantly. Free floating sections could be incubated for longer time in blocking buffer (with 0.5% Tritonx-100).
Specific staining, good for thinner sections and thicker slices.
Antigen retrieval is required for enhanced labelling.
Specific signal