CD44 Antibody for IHC

September 08, 2020

Radiation Oncology
UAB
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling

Product Name:

CD44 (E7K2Y) XP® Rabbit mAb

Catalog Number:

37259

Clone Number:

N/A

I measured CD44 by immunohistochemistry and observed an interesting trend with treatment in human breast cancer cells. CD44 was used as a cancer stem cell marker.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human breast cancer cells

Primary Incubation

1:200

Blocking Agent

5% BSA

Secondary Incubation

1 hour at RT

Tertiary Incubation

1:1000

Detection

Tyramide Signal Amplification.

Results Summary

Signal was excellent with tyramide signal amplification. I saw a significant change in expression with treatment with HDAC inhibitor in human breast cancer cells.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very good signal can be obtained.

The Bad

N/A

The Bottom Line

This CD44 antibody should be used for total CD44 expression. However, other isoforms of CD44 due to splicing variants may be observed. In this case, other antibodies should be used to specifically observe those variants.

Share your experience with other scientists. Write a Review! »

Join the discussion