Abcam
Anti-PCNA antibody
ab18197
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E13 embryonic mouse brain tissue was stained with anti-PCNA to determine the total proliferating cells in the cortex.
Immunofluorescence
Frozen embryonic mouse brain tissue (E13)
1:800, 4 degrees overnight
10% Normal donkey serum in PBST (0.5% Triton X 100)
1:500, AF 488
None
Proliferating cells were specifically marked with the antibody. A few cells show punctate staining with the antibody. these are the cells in the S-Phase of the cycle, hence one can also determine the total S-phase cellular population in a specific tissue.
Antigen retrieval in 10mM Sodium citrate buffer (pH 6) is required for improved staining.
Specific staining, good for thin sections.
Antigen retrieval may be necessary for better staining, concentrated antibody may give unspecific signals.
Recommended.