Miltenyi Biotec
IgM Antibody, anti-mouse, VioBlue®, REAfinity™
130-116-215
REA979
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Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) in health and disease. In this particular experiment, we are looking for IgM-expressing B cells.
Flow Cytometry
Mouse spleen
Anti-mouse IgM VioBlue 1/25, 15', 4°C
Fc block (2.4G2 clone)
NA
Flow cytometer
We could very nicely stain IgM+CD19+ B cells.
VioBlue can be read in the same channel than BV421.
It works well
It can be used