Rabbit Anti-Histone H3-phospho (Ser10) Works Well

August 26, 2020

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Rabbit anti -Histone H3-phospho (Ser10)

Catalog Number:

9701

Clone Number:

N/A

Good antibody to detect cells in mitotic division (red). High specificity and low background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryo E11.5: Spinal cord

Primary Incubation

1:200, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

Good quality antibody to detect cells in division (red). Low background staining.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Quality.

The Bad

None.

The Bottom Line

Good quality antibody to label cells in mitotic division. Recommended.

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