Rabbit Anti-CD45 Antibody for Immunofluorescence

September 14, 2020

Angioscience
ECAS
Graduate Student

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Company:

Abcam

Product Name:

Rabbit Anti-CD45 antibody

Catalog Number:

ab10558

Clone Number:

N/A

This antibody detects CD45+ cells with specificity. A considerable background is present in some samples.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rabbit Anti-CD45 antibody

Primary Incubation

1:100, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

This antibody detects CD45+ cells in the adult spinal cord, where a certain background staining is observed.

DOI or PMID #

N/A

Additional Notes

N/A

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The Bottom Line

A good antibody to be used to identify CD45+ cells.

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