Rabbit Anti-Iba1 Antibody for Immunofluorescence

September 17, 2020

BlueskyReddit
Angioscience
ECAS
Graduate Student

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Company:

Wako

Product Name:

Rabbit Anti-Iba1 antibody

Catalog Number:

019-19741

Clone Number:

N/A

Antibody detects macrophages (during development) and microglia (adult) correctly with little background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Adult mouse spinal cord

Primary Incubation

1:300, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Tertiary Incubation

None.

Detection

Confocal microscope.

Results Summary

This antibody correctly detects macrophages during spinal cord and brain development.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Quality.

The Bad

None.

The Bottom Line

This antibody is highly recommended.

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