Rabbit Anti-beta-Tubulin III for Immunofluorescence

September 09, 2020

Angioscience
ECAS
Graduate Student

Overall

Quality of Results

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Company:

Millipore Sigma

Product Name:

Rabbit Anti-beta-Tubulin III

Catalog Number:

T2200

Clone Number:

N/A

Antibody used to detect newly axons/neuronal area in the developing spinal cord.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryo E11.5: Spinal cord

Primary Incubation

1:300, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS

Blocking Agent

2%Normal donkey serum in 0,3% triton-x100 in PBS

Secondary Incubation

2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PB

Tertiary Incubation

Not done.

Detection

Confocal microscope.

Results Summary

This antibody labels the differentiated area of the developing spinal cord with little background.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Quality.

The Bad

Nothing.

The Bottom Line

This antibody is recommended for researchers looking to label axons during development.

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