Recommended: NEFM Monoclonal Antibody

August 07, 2020

Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

Thermo Scientific

Product Name:

NEFM Antibody

Catalog Number:

13-0700

Clone Number:

N/A

20µm cryosections E13 mouse brain embryonic tissue were used to determine and label the axons from the newly generated pyramidal neurons of the neocortex

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Frozen embryonic mouse brain tissue (E13)

Primary Incubation

1:200, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Axonal fiber staining from the newly generated neurons of the cortex is distinctively labelled in the intermediate region of the embryonic cortex.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific staining, good for thin sections.

The Bad

Penetration in sections thicker than 100µm is not possible

The Bottom Line

Recommended for labelling the medium neurofilament chain. However, remember that it does not label all the axons of the neocortex.

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