Cell Signaling Phospho-Histone H3 (Ser10) Antibody Works Well

August 06, 2020

BlueskyReddit
Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

Cell Signaling

Product Name:

anti-Phospho-histone H3

Catalog Number:

9701

Clone Number:

N/A

20µm cryosections or 500µm thick slices of E13 mouse brain embryonic tissue were used to determine and label the actively dividing population of progenitors of the neocortex.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Frozen embryonic mouse brain tissue (E13) and thick slices of 500 microns

Primary Incubation

1:500, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF647

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

Actively dividing mitotic cells were labelled in the apical as well as in the intermediate position of the E13 mouse neocortex.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good for thicker sections and brain slices also. whole brain staining is possible due to excellent penetration depth.

The Bad

Sometimes a dilution lesser than 1:500 ( more concentrated antibody) may give some unspecific staining.

The Bottom Line

Highly recommended when used at proper dilution and standardization.

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