Great Purified anti-Pax-6 Antibody

August 06, 2020

BlueskyReddit
Vascular Dysfunction
University of Heidelberg
Postdoctoral Researcher

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Company:

Biolegend

Product Name:

anti-Pax6

Catalog Number:

PRB-278P

Clone Number:

Poly19013

Mouse embryonic brain sections E13 (fixed and frozen-20µm) were used to determine the proportion of Pax6 positive apical progenitors in the neocortex.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Frozen embryonic mouse brain tissue (E13)

Primary Incubation

1:200, 4 degrees overnight

Blocking Agent

10% Normal donkey serum in PBST (0.5% Triton X 100)

Secondary Incubation

1:500, AF 488

Tertiary Incubation

None

Detection

Immunofluorescence

Results Summary

The apical progenitors were specifically labelled using this antibody.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Specific staining, no cross reactivity to neurons and other intermediate progenitors, easy to use.

The Bad

None

The Bottom Line

Recommended

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