AVES Labs
Anti-Nestin
NES
Info:Supplier Page
Browse Similar Products: Anti-nestin Immunofluorescence Antibodies
E11 Frozen embryonic mouse brain sections were blocked with (10% Normal donkey serum in PBST (0.5% TritonX 100)). Primary antibody (1:1000) was used in the same buffer as blocking and the sections were incubated at 4 degrees O/N. fluorescently conjugated AF488 secondary was added after 3 washes of PBS (10 min each) and incubated for 2h at RT. Slides were washed and mounted using fluormount.The antibody does not work well with thick vibratome sections upto 250 µm as the penetration of the antibody is not good.
Immunofluorescence
Frozen embryonic mouse brain cortex tissue (E11)
1:1000, 4 degrees overnight
10% NDS in PBST
1:500, AF 488
None
The antibody is highly specific and works in 20µm thin sections. radial gial fibers are visible in the image that run parallel from apical to basal area of the neocortex.
Specific staining, good for thin sections.
Penetration in sections thicker than 50µm is not possible.
Recommended for immunostaining up to 25µm frozen or free floating sections.