Sigma
Beta Tubulin Isotype III (clone SDL.3D10)
T5706
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Frozen embryonic mouse brain sections were blocked with (10% Normal donkey serum in PBST (0.5% TritonX 100)). Primary antibody (1:400) was used in the same buffer as blocking and the sections were incubated at 4 degrees O/N. fluorescently conjugated AF488 secondary was added after 3 washes of PBS (10 min each) and incubated for 2h at RT. Slides were washed and mounted using fluormount.The antibody also works well with thick vibratome sections upto 250 µm as the penetration of the antibody is good. The incubation period could be one day longer.
Immunofluorescence
Frozen embryonic mouse brain tissue
1:400, 4 degrees overnight
10% NDS in PBST
1:500, AF 488
None
The antibody is very specific and stains nascent neurons completely. At early embryonic stages when the neurons are just born, little axonal tracts are also visible.
N/A
Specific staining, good for thicker sections and brain slices.
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