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PerCP Cy5.5 anti-mouse CD3e antibody
100328
145-2C11
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It is well known that B lineage cells play a major role in pathogenesis of autoimmune diseases through presentation of antigen to T cells, production of autoantibodies and inflammatory cytokines that modulate immune responses. We are interested in elucidating the biology of B and T lineage cells during autoimmunity. In this experiment, I want to stain T cells (CD3+).
Flow Cytometry
Wild-type mouse spleen
Anti-CD3 Percp-Cy5.5(1/200) (20 min, 4° in the dark)
Clone 2.4G2 (Fc block)
N/A
Flow cytometry, blue laser
I was able to stain CD3 T cells but the signal was not very bright.
The antibody works
Not very bright
It works okay for staining CD3 in T cells.