Great Smad2/3 Antibody Sampler Kit

July 14, 2020

BlueskyReddit
Pharmacology
Duke University
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Cell Signaling Technologies

Product Name:

Smad2/3

Catalog Number:

12747T

Clone Number:

N/A

Worked really well. Fibronectin was detected in human fibroblast stimulated with TGFB. Cells were lysed and ran through western blot for expression analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse

Primary Incubation

1:1000

Blocking Agent

NDS

Secondary Incubation

1:1000

Tertiary Incubation

N/A

Detection

Chemiluminescence

Results Summary

Fibronectin was detected in human fibroblast stimulated with TGFB. Cells were lysed and ran through western blot for expression analysis.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

This antibody detected one single band at the correct weight.

The Bad

Optimization of incubation times is needed.

The Bottom Line

This product provides a good smad2/3 band.

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