Great Signal with BioLegend's CD34 Antibody

July 17, 2020

Experimental Hematology
Department of Biomedicine, University Hospital Basel
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Pacific Blue™ anti-human CD34 Antibody

Catalog Number:

343512

I used this antibody to FACS stain PBMC sample from patients. I used the concentration specified by the company and stained the cells at RT for 30 minutes post Fc Block.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

1 ug/million cells, Room Temperature, 30 minutes

Blocking Agent

Human Fc Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry

Results Summary

Analysed the expression of CD34 on peripheral blood HSCs and HSPCs from patient PBMC samples.

Image Gallery

Summary

The Good

Very good signal

The Bad

None

The Bottom Line

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