IL6 ELISA kit from Biolegend Worked Great

Brigham and Women's Hospital and Harvard Medical School
medicine
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

ELISA MAX™ Deluxe Set Human IL-6

Catalog Number:

430504

We are working on bacterial infection and host innate immunity. We detect the human macrophage responses to bacterial vesicles, and used this kit to measure the IL-6 production, a marker of NF-kB activation.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Human macrophage supernatant.

Tips

None

Results Summary

To test whether the kit works well, we treated human macrophages with TLR2 ligand Pam3CSK4, and tested the IL-6 from culture supernatant of human macrophages. As expected, Pam3CSK4 but not untreated cells, significantly induced IL-6 production

DOI or PMID #

N/A

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Summary

The Good

Sensitive, specific, and cheap

The Bad

None

The Bottom Line

I give highest recommendation for this ELISA kit.

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