Great Thermo Fisher Scientific Permeabilization Buffer for Flow Cytometry on Human Cells

COLUMBIA UNIVERSITY
M&I
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Permeabilization Buffer 10X

Catalog Number:

00-8333-56

Our lab studies the behavior of T cells during activation and to do so we look at the expression of transcription factors. In order to stain for the transcription factors, we need to be able to permeabilize our cell samples and this buffer allows us to do so.

Experimental Design and Results Summary

Application

Flow cytometry

Starting Material

Human lymphocytes

Tips

Dilute 10:1

Results Summary

This permeabilization buffer works very well with our samples to allow for staining.

DOI or PMID #

N/A

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Summary

The Good

Easy to use.

The Bad

None.

The Bottom Line

This is an easy permeabilization buffer to use with T cells to prepare cells for intracellular staining.

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