Abcam
NAD/NADH Assay kit (colorimetric)
ab65348
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This NAD/NADH assay kit supports material like urine, serum, or lysates, and most importantly gives a quantitative NAD/NADH ratio. I use it for testing the NAD/NADH ratio in tissues and serum, which requires precise purification.It is relatively convenient and easy to optimize, with the capability to quantify NAD and NADH individually or their ratio, and have no background counting from NADP or NADPH.The best part of this kit is if your sample will have autofluorescence, it does not read it. The drawback is the sensitivity of this method since a colorimetric assay was used, it is not as sensitive as a fluorometric assay.
Ratio in mouse tissue and serum.
Mouse with different treatment.
Do your calculations for your standard curve ahead of time; flash freeze your samples if cannot be done in the same day.
I want to see if there is any difference in NAD/NADH between control and treated mice, I collected tissue and serum from mice, flash freeze them, and then the second day I processed them with this kit. The result shows a relatively good number comparing to several publications.
No fluorescent interference; detailed protocol provided in the kit.
Expensive, procedure is very long.
NAD/NADH ratio is hard to measure, this kit gives a good result for tissue and serum.