Good Option to Stain Human CD4

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

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Company:

BD Biosciences

Product Name:

BUV395 Mouse Anti-Human CD4

Catalog Number:

563552

Clone Number:

SK3

Info: Supplier Page

Browse Similar Products: CD4 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody- secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining B cells (CD19+) and CD4+ T cells in isolated PBMCs from peripheral blood of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Healthy donor PBMCs

Primary Incubation

anti-CD4 BUV395 1/20, 15' 4°C

Blocking Agent

FC block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

We can clearly stain CD4 T cells.

Image Gallery

Summary

The Good

It works well

The Bad

It is outrageously expensive!

The Bottom Line

It works!

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