Very Good Anti-human CD27 Antibody for Flow Cytometry

B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

FITC Mouse Anti-Human CD27

Catalog Number:

555440

Clone Number:

M-T271

Info: Supplier Page

Browse Similar Products: CD27 Flow Cytometry Antibodies

Antibodies are produced by plasma cells (PC) that are terminally differentiated B cells and play crucial roles in host protection against infections but are considered to mediate autoimmunity in patients with rheumatic diseases, such as systemic lupus (SLE) or rheumatoid arthritis (RA). Our research aims at understanding the biology of antibody-secreting cells (ASC) and B cells in health and disease. Here, we aimed at staining memory B cells (CD20+CD27+) in whole blood (WB) of a healthy donor (HD).

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human WB

Primary Incubation

anti-CD27 1/25 15' 4°C

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometer

Results Summary

We could very nicely stained memory B cells CD20+CD27+.

Image Gallery

Summary

The Good

It works well!

The Bad

NA

The Bottom Line

Good option to stain human CD27

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