Detecting EGR1 Expression in CD4+ T Cells

July 03, 2020

Microbiology and Immunology
Columbia University Irving Medical Center
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Cell Signaling Technology

Product Name:

EGR1 (44D5) Rabbit mAb (PE Conjugate)

Catalog Number:

25357

Clone Number:

44D5

Our lab studies T cell biology and is examining TCR induced activation profile in T cells. There was expected increase in Egr1 expression upon overnight stimulation of CD4 T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse, splenocytes

Primary Incubation

1:50, 30 mins on ice

Blocking Agent

none

Secondary Incubation

none

Tertiary Incubation

none

Detection

BD LSRII

Results Summary

Upon in vitro activation of CD4 T cells overnight, there is increase in Egr1 expression (red).

Image Gallery

Summary

The Good

Worked well

The Bad

na

The Bottom Line

Recommended antibody and can be used for activation of mouse T cells

Share your experience with other scientists. Write a Review! »

Join the discussion