TCF1/TCF7 Rabbit mAb from CST Works Well on T Cels

July 15, 2020

Microbiology and Immunology
Columbia
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

TCF1/TCF7 (C63D9) Rabbit mAb

Catalog Number:

6709

Clone Number:

C63D9

Used it on mouse lung and draining lymph node T cells, after flu infection and it worked well. Was able to examine expression in naive (higher) and effector (lower) T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse

Primary Incubation

1:50

Blocking Agent

None

Secondary Incubation

none

Tertiary Incubation

none

Detection

LSRII

Results Summary

Expression was great in both naive and effector T cells with naive T cells having higher expression. Fluorophore is also stable and good one to use

Image Gallery

Summary

The Good

Good staining

The Bad

na

The Bottom Line

Great antibody, highly recommended.

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