Great Qiagen Buffer for Lysing Cells

COLUMBIA UNIVERSITY
M&I
Lab Director / Manager

Overall

Quality of Results

Ease-of-Optimization

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Company:

Qiagen

Product Name:

Buffer RLT Plus (220 ml)

Catalog Number:

1053393

Our lab studies T cell subsets in various human tissues and their function. One of our projects is to extract RNA from sorted cells for analysis, and this allows us to both lyse the cells for extraction and keep the RNA stable in the freezer.

Experimental Design and Results Summary

Application

Cell lysis

Starting Material

Sorted human cells

Tips

Need to add BME

Results Summary

This lysis buffer easily breaks the cells to prepare for RNA extraction.

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

This lysis buffer prepares our cells for RNA sequencing and keep them stable for -80 freezer storage.

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