Good Anti-CD45RA Antibody

July 06, 2020

BlueskyReddit
B cell memory
Deutsches Rheuma-Forschungszentrum, a Leibniz Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PE/Cyanine7 anti-human CD45RA Antibody

Catalog Number:

304126

Clone Number:

HI100

It is possible to distinguish between CD4+CD45RA- memory and CD4+CD45RA naive T cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Healthy donor PBMCs CD8 depleted

Primary Incubation

anti-CD45RA PE-Cy7 1/100 20', 4°C

Blocking Agent

Fc block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry

Results Summary

It is possible to distinguish between CD4+CD45RA- memory and CD4+CD45RA naive T cells.

Image Gallery

Summary

The Good

It works

The Bad

NA

The Bottom Line

It is a nice option to stain CD45RA!

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