Efficient Reagent for Difficult to Transfect Cell Lines

Johns Hopkins University School of Medicine
Department of Cell Biology
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Lonza

Product Name:

Cell Line Nucleofector Kit V

Catalog Number:

VCA-1003

We used this kit for efficient transfection of difficult to transfect cell lines. I used different cell types such as HL-60, MDA-MB-231, and RAW 264.7.

Experimental Design and Results Summary

Application

Nucleofection

Starting Material

Human cell lines

Tips

Perform the experiment according to manufacturer's instructions.

Results Summary

Transfection efficiency is very high and works well. But for some of cell lines such as HL-60 cells, there are lots of dead cells after the transfection. Cancer cells can survive well after the transfection.

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Summary

The Good

Reliable, high quality, user friendly

The Bad

The price is high and you need to finish the experiment very soon

The Bottom Line

I highly recommend this for Nucleofection. It works very well.

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